We introduced this novel biolipid assay of the isoprostane level in dogs in May 2019.

To re-iterate, Hemopet’s CellBIO assay quantitates the isoprostane level in dog saliva to determine if the pet’s body is undergoing harmful cellular oxidative stress. Cellular oxidative stress creates reactive oxygen species (ROS) causing cells to undergo damage and release biomarker lipids and enzymes that lead to tissue inflammation, infections, obesity and even cancers. This is known as dysbiosis.

Healthy cells, in contrast, are in symbiosis (harmony) within the body and are maintained in this state primarily by the presence of a healthy, balanced microbiome. This microbiome is first present within the body during fetal development, and at birth, and then continues to function throughout life.

The microbiome organisms (microbiota) that reside in the gut (bowel) are the most diverse and abundant, and so we need to focus on the health benefits of the microbiome, such as by providing probiotics. The benefits of this approach are easy for the consumer to observe and measure not only in themselves, but also in other people and pets.

Observable benefits include lessening in the severity of eczema and other skin disorders, improved protein digestion—measurable by absent or reduced intestinal gas—in young human and animal athletes that consume protein-rich diets, and the alleviation of constipation in senior people and aging pets. These benefits improve the quality of life across all life stages.

However, these benefits are contrasted with the harmful effects of a disrupted gut microbiome (dysbiosis). It creates an unhealthy gut barrier that can trigger cellular inflammation—which is directly related to imbalances in the important neurotransmitters of the gut-brain axis. This in turn contributes to the cognitive and memory decline associated with aging and even to more serious issues such as depression. Yes, animals can experience depression too.

The good news about this give-and-take balance of microbiota is that balancing the gut microbiome in a healthy manner with selected nutrients and supplements can help counteract this cellular oxidative stress and ROS production. One proven way this can be accomplished is with so-called functional foods that activate the body’s critical Nrf-2 pathway: e.g. turmeric, chili pepper, ginger, green tea, soybeans, tomatoes, berries, raw honey (not for the very young), garlic in moderation, coconut, cabbages, and broccoli. The newly coined term for these foods is Bioceuticals, a holistic approach to improving cognitive health and longevity.

Update on CellBIO Results

After completing the initial clinical trial studies, we have analyzed 116 clinical patient samples; 27 of them were positive, having isoprostane biolipid levels above the normal reference range we have established (i. e., 0.5-1.75 ng/mL of saliva).

Of the 27 positive testing dogs, there were: 10 spayed females, 9 neutered males, 6 intact females, and 2 intact males. The ages ranged from 9 months to 12 years, although most were middle aged or older. The weight range was 9-90 pounds, and no breed type predominated.

Note that the sex breakdown and ages could merely reflect increased choice of testing as older pets are more likely to be neutered or spayed and have some health issues.

We have recommended that the positive testing dogs be retested in about 6 months after being on foods and supplements designed to lower their cellular oxidative stress. We anticipate that a positive outcome will reveal reduced isoprostane levels when retested.